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Trouble shooting western blott

Trouble shooting western blott - Basic Lab Protocols and Techniques

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  #1  
Old 08-14-2006, 09:25 PM
Pipette Filler
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Default Trouble shooting western blott



Dear Friends
Ia m facing a problem with non specif reactivity with proteins in western blott and I need importnat information on the siluti=on used for antibody solutions
Iam preapraing my primary antibody in 2 percent milk pwder is it right ?????
Shall I use PBS to prepare my primary and secondary antibody ?????
Please send rely soon
Vivek
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Old 08-15-2006, 10:38 PM
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Default Re: Trouble shooting western blott

Hey dude,
use TBST I think it may help... 5% milk is better as well
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