dna plant/mammal ## osmoregulation

[algory]

Hey folks:
I did DNA precipitation using ethanol from about 8g liver [L] and fruit tissue [F] lysate.
Result: recovered very huge yield of dna/protein-complex in L compared to F. Furthermore in L- tube the precipitate was located in the interphase as a 'lump' whereas in the F-tube the DNA ascended to the surface after precipitation. F-precipitate had a fibrous shape.

Conclusion:
L: high Protein rate due to higher acticity (proteinbiosynthesis) which makes the precipitate more heavy than the F-one?
F: low activity, less protein, "clean" DNA ascenced to surface?

Questions:
- does someone has a reference for my observations?
Can't find literature which tells me the amount of dna/protein/polysaccharides etc. in the cells -> can't proof my conclusion.
- reference to where the cell-components are located?

- lower phase = sds -> contains cell wall, polysaccharides...?
- upper phase = ethanol -> contains membrane ?
-> correct? why?


'''''''''''''''''''''''''''''
looking for literature to osmosis in red blood cells placed in hyper/hypotonic NaCl solutions.
What is the generall molarity in the red blood cells?
detailled information to the regulation?

Found only informations to the structure and function of erythrocytes.



I appreciate every explanation you can give me to the experiments

regards,
al