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11-30-2007, 08:16 PM
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 Elisa Hi, I would like to know if ELISA can be used as a simple spectrophotomer to analyze a large number os samples for lipid and carbohidrate determinations. A friend of mine is convinced that it is possible but I have some dubts, but I do not have any experience in the use of this instrument. Thanks in advance. milpol        |
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11-30-2007, 08:36 PM
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 Re: Elisa The ELISA is just a 96-well plate format for conducting an enzyme-linked immunosorbent assay, which undergoes a chromogenic reaction that is read by a simple spectrophotometer. Therefore the same spectrophotometer VIS or UV/VIS combo can be used for other absorbance readings (i.e. non-ELISA assays) like protein quantitation, OD260/280 DNA/RNA absorbance ratios etc. |
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