lysis by sds

[algory]

Hello!

Im familiar with the effect of SDS on cell membranes.
Now I'm looking for an exact explanation for the SDS-protein interaction which turns the membrane "leaky" (if possible with images)
References (publications, books...) are also very important for me.

thanks in advance

regards,
al

[danfive]

I've linked to this previously--->

Originally Posted by danfive

For more background you may want to read "A Guide to the Properties and Uses of Detergents in Biology and Biochemistry" by Srirama M. Bhari
--> http://www.biology.ucsd.edu/classes/...oklet_2001.pdf

Here's a quote p.8-9
“Dissolution of membranes by detergents can be divided into different
stages (Figure 6). At low concentrations, detergents bind to the membrane
by partitioning into the lipid bilayer. At higher concentrations,
when the bilayers are saturated with detergents, the membranes disintegrate
to form mixed micelles with the detergent molecules. In the detergent-
protein mixed micelles, hydrophobic regions of the membrane proteins
are surrounded by the hydrophobic chains of micelles. In the final
stages, solubilization of the membranes leads to the formation of mixed
micelles consisting of lipids and detergents and detergent micelles containing
proteins (usually one protein molecule per micelle).”

I also attached the figure 6 mentioned above.

[algory]

Thanks for reply!
Great publication!

regards,
al

[algory]

Hey!

Found the "ultimate" reference:

Noll, H., and E. Stutz. 1968.
The use of sodium and lithium dodecyl sulfate in nucleic acid isolation,
p. 129-155. In S. P. Colowick and N. 0. Kaplan (ed.),
Methods in enzymology, vol. 12B. Academic Press
Inc., New York.


regards,
al