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Help with reagents in RNA purification

Help with reagents in RNA purification - Basic Lab Protocols and Techniques

Help with reagents in RNA purification -

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Old 11-11-2007, 11:40 PM
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Default Help with reagents in RNA purification

Dear friends,

this is my first time and ny first post in this forum.
I would appreciate if someone could help me. I need to know what is the role of every reagent used in the classical isothiocyanate–phenol:chloroform extraction protocol used in RNA purification.
Here are the reagents:

4 M guanidine isothiocyanate (GITC)
0.02 M sodium citrate
0.5% sarcosyl
2 M Sodium acetate (pH 4.0)
Chloroform:isoamyl alcohol
Phenol (equilibrated to pH 5.3–5.7 with 0.1 M succinic acid)
β-Mercaptoethanol (β-ME) (14.4 M)
Glycogen (2 mg/ml)
Diethylpyrocarbonate (DEPC)
DEPC-treated water
75% (v/v) ethanol (prepared using DEPC-treated water)

All the best and thanks a lot to everybody.
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purification , reagents , rna

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