gelatin zymography problem

[monosyllabikgurl]

Hi,
Just started running gelatin zymograms but the gelatin is getting electrophoresed even though it is run at 10 mA per gel in the fridge on ice. Ending up with half the gel dark background at the upper half light so there is not much contrast with the positive signals. Have changed the APS and acrylamide as well as changing the gelatin size in the gel and nothing has worked.

Has anyone experienced this and has any advice?

Thanks!

[labgnome]

what percentage of gelatin are you using? 0.1% works perfectly.

[monosyllabikgurl]

Yes we have been using 0.1%. Nothing seems to work. And someone else in the lab did tons of these a couple years back and never had any problems. And I'm using her protocol. . .

[nagakiran]

hi guys can u give me the material source of this gelatin zymography