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09-18-2007, 09:47 AM
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 washing of phosphorylated plasma protein Analytical chemist with little experience of plasma and proteins need help with: What buffer should I use to wash a protein pellet (precipitated from plasma with 35% ACETONE/WATER) to ensure that all free and non-protein bound phosphate from the plasma residual is removed while protein bound (especially serine-phosphorylated) phosphate is not released? (I thereafter want to quantify the alkali-labile protein bound phosphate) many thanks..        |
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