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yeast transportation - Basic Lab Protocols and Techniques

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  #1  
Old 09-06-2007, 03:21 PM
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Smile yeast transportation



Hi
i want to send yeast strain to my friend living abroad, what is the best way to send yeast strain so that they also dont loss their viability.
i hope you will guide me soon.


best regards
aftab ahmad
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Old 09-10-2007, 12:21 PM
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Default Re: yeast transportation

Hi there Aftab,

I would recommend making a stab. To to this:

1. Make a small 10-20mL stock of sterile YPD agar (or similar).
2. Transfer the agar into a 30-50mL sterile tube (e.g. a Falcon tube)
3. Keep the tube at a 45 degree angle until the agar sets
4. Take some of the culture onto an innoculation loop and stab it deep into the center of the agar.

The culture should be fine in there for several weeks at room temperature. When your friend get the culture he/she should stab an innoculation loop into the agar and use it to break up the agar, then streak onto fresh plates.

Last edited by Bitesizebio; 09-10-2007 at 12:24 PM.
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Old 09-10-2007, 07:10 PM
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Default Re: yeast transportation

Hi Dear
Thanks for your guidence
this is not only my problem and we should discuss this topic in detail so i would like that other members should also contribute and also give some other way for bacterial and yeast transportation.
regards
aftab
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