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Large Scale Extraction of Bacteria Plasmid DNA

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Old 06-16-2007, 08:53 PM
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Default Large Scale Extraction of Bacteria Plasmid DNA
Please tell me the usage of GTE buffer, NaOH/SDS, potassium acetate, 70% ethanol, 95% ethanol and isopropanal in extraction of bacteria plasmid DNA.
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Old 06-18-2007, 11:12 AM
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Default Re: Large Scale Extraction of Bacteria Plasmid DNA
In brief: Cell Lysis- Protein denaturation- precipitation of chromDNA, ppt. of plasmid DNA last step purifiication step.
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Old 06-18-2007, 07:52 PM
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Default Re: Large Scale Extraction of Bacteria Plasmid DNA
Can you please tell me the details of them. I need to do a report. Thanks
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Old 06-18-2007, 08:05 PM
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Default Re: Large Scale Extraction of Bacteria Plasmid DNA
Try this site to help you get started. You might want to look up each step individually in more detail -- she gives an idea of what's happening, but you might try to find some old papers that explain the why.
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bacteria , dna , extraction , large , plasmid , scale

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