protein molecular weight determination

[vijaya]

Hi all,
iam new to this work
how 2 determine protein molecular weight using RF values
thanks in advance

[ESHWAR]

Hi
You need some standards. The standards are those whose molecular weights are known. Obtain their RF values and plot the standard graph, with RF value vs molecular weight. Now mark the RF value of your unknown protein, and you can determine its molecular weight.

[pluto]

actually, the best way to do it is to do a Ferguson plot. You have to run you standards and your protein of interest on 3-4 gels that are different acrylamide concentrations, then do a bunch of plots.

Do a search for Ferguson plot and you will find it