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protein molecular weight determination

protein molecular weight determination - Basic Lab Protocols and Techniques

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Old 04-29-2007, 02:46 PM
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Default protein molecular weight determination



Hi all,
iam new to this work
how 2 determine protein molecular weight using RF values
thanks in advance
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Old 06-22-2007, 01:38 PM
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Lightbulb Re: protein molecular weight determination

Hi
You need some standards. The standards are those whose molecular weights are known. Obtain their RF values and plot the standard graph, with RF value vs molecular weight. Now mark the RF value of your unknown protein, and you can determine its molecular weight.
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Old 11-15-2007, 06:42 AM
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Default Re: protein molecular weight determination

actually, the best way to do it is to do a Ferguson plot. You have to run you standards and your protein of interest on 3-4 gels that are different acrylamide concentrations, then do a bunch of plots.

Do a search for Ferguson plot and you will find it
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