Problems with Invitrogen?

[steveF]

Hello.

Fot the past few weeks I have been trying to amplify several fragments using Invitrogens platinum pfx. I got very little success, either no amplification at all or very faint bands. This was using template/primer pairs that I had shown to work with taq. After changing everything, I finally did a definitive exp comparing the pfx against pfu-ultra and herculase. both of the latter gave good strong single bands. pfx gave nothing at all.

This is not the first time i have had problems with invitrogen products. I simply do not believe the claims that they make for their products or their claims of qualit control.

Avoid pfx platinum. It is a very poor product.

Steve