Originally Posted by admin
The best way to visualize and keep things safe (and easy disposal) is to incorporate the ethidium bromide inside the gel (after the microwaved gel cools a bit).
That's the way we do it and because we always have done it this way I have never seen the difference in visualization of electrophoretic results.
Once I read that ethidium bromide incorporated into the gel prior to electrophoresis gives less sharp/less visible bands ,or something like that, comparing to staining the gel after electrophoresis in ethidium bromide solution.
So, apart the differences in handling, safety and disposal, what's the difference between the two staining approaches in results obtained?
I've watched many vids where the gel is stained with ethidium bromide after elecrophoresis - I consider it less safe. If it is so, why is the method used? What are its advantages?