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Ethidium bromide

Ethidium bromide - Article Discussion

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  #31  
Old 09-05-2010, 10:39 AM
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Default Re: Ethidium bromide

thank you very much sharda
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  #32  
Old 10-15-2010, 06:32 AM
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Default Re: Ethidium bromide

This conversation is going no where. Itís lacking the place of a good leader to
head the things to come out on conclusion.
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  #33  
Old 10-17-2010, 05:40 PM
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Post Re: Ethidium bromide

The basic problem with this discussion is that nobody is trying to discuss EtBr use.
One problem with EtBr in most of the scientific discussions is whether to use it for nucleic acid band detection or not. Some say, add it to gel during solidification, some say do external staining while some say omit its use.
Because of efficiency and feasibility, most people don't want to omit it and there is also not a good alternative for it.
For using it, many people question its carcinogenicity. But according to my knowledge (may be I am wrong), nobody has proven its in vivo carcinogenicity. At least no such research paper I have found.
I think our discussion should aim minimum and maximum EtBr concentration required for DNA detection and also what is good procedure for elution of band detected by EtBr.
If anybody come forward then we can get fruitful discussion.
I am not a born scientist and not most of others. We r here in this forum to enrich our knowledge and according to me, this discussion now require a new path, it is not an end.
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  #34  
Old 10-19-2010, 12:02 AM
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Default Re: Ethidium bromide

Well said. I'm also interest it in learning more about EtBr.
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  #35  
Old 10-19-2010, 02:50 PM
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Default Re: Ethidium bromide

Quote:
Originally Posted by Aga View Post
I found out that EtBr boils at 100 degrees C. This was stated on BioRad product description.
So it seems it may be dangerous when heated and/or added to hot agarose before casting the gel.
Ethidum bromide does NOT boil at 100C. Biorad sells only ethidium bromide solution so it's the aqueous solution that boils, not ethidium bromide itself. EB melts at >260C at which it decomposes, so I would say there's negligible ethidium bromide vapour. However, EB solution will be aerosolised during boiling, so you may want to let the solution stand before you open it. Even then, the amount of EB in the aerosol will be minute since most of it (> 99.9% ?) will remain in the solution.
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  #36  
Old 02-24-2011, 06:32 AM
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Default Re: Ethidium bromide

The best way to visualize and keep things safe (and easy disposal) is to incorporate the ethidium bromide inside the gel (after the microwaved gel cools a bit).
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  #37  
Old 03-09-2011, 09:25 PM
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Default Re: Ethidium bromide

ETbr is great except its always over used!! I always can get away with using half of what they say to. Also try to make just enough agrose for one gel bc reheating agrose with ETbr inside allows for inhalation and probably isn't that great in the long run
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  #38  
Old 03-09-2011, 09:27 PM
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Default Re: Ethidium bromide

Also run a agrose gel +etbr and then go ahead and run a second gel in the same buffer means you dont have to add etbr to the new batch or add .5ul for 100ml(agrose). It sticks around in the buffer, which it means its sticks around in lab!!
lets try to use as little of this awesome but dna strand breaking lab tool
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  #39  
Old 06-15-2011, 04:53 AM
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Default Re: Ethidium bromide

thanks for the info sir...


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  #40  
Old 09-14-2012, 02:26 AM
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Default Re: Ethidium bromide

EB fluorescence could be excited not only with UV, but with the Vis light as well.
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