Originally Posted by Aga
I found out that EtBr boils at 100 degrees C. This was stated on BioRad product description.
So it seems it may be dangerous when heated and/or added to hot agarose before casting the gel.
Ethidum bromide does NOT boil at 100C. Biorad sells only ethidium bromide solution
so it's the aqueous solution that boils, not ethidium bromide itself. EB melts at >260C at which it decomposes, so I would say there's negligible ethidium bromide vapour. However, EB solution will be aerosolised during boiling, so you may want to let the solution stand before you open it. Even then, the amount of EB in the aerosol will be minute since most of it (> 99.9% ?) will remain in the solution.