| | Re: Ethidium bromide
I realize I'm late coming to this thread, but I thought I'd share a couple of things we've found in our lab with the syber and etbr dyes.
Firstly, we've found that staining with SYBR seems to interfere with some restriction enzymes and with T4 DNA ligase. As such we've stopped using it for digests/ligations because of the apparent incompatibility. Invitrogen claims SYBR works fine for these applications, but at least in our hands, it seems to interfere.
Secondly, for those worried about disposal, you can (or at least could) get filters that would bind up free EtBr. Simply place over a beaker, pour the solution through, and you get EtBr "free" run-through which can be disposed of the same as any other water-miscible waste. The filter goes to hasmat.
Lastly, we've found that not all equipment setup for EtBr works for SYBR (due to differences in emission wavelength). So while switching may make the lab safer (or at least, gels easier to run), you may need to upgrade some of your equipment.