- Protein concentration
- Normalizing samples
- Migration problem
- Bradford-protein problem
- Special blocking procedure/product for using mouse antibody on mouse tissue
- Antibody raised in mouse used on mouse tissue?
- alpha 1 a
- problem with WB quantification
- Brick or blank blot
- Detection of gamma H2AX
- protein stuck
- GFP antibody
- Loading Control Calculations
- cytoplasmic and nuclear protein extration
- right way to assemble the sandwich
- Strong signalling that disappears fast
- Infected Cells Have Lighter Bands than Control
- Has anyone tried the LUMINOLPEN
- Can ice-pack be a problem?
- Tricine running buffer
- Correcting for protein loading.
- Ladder issue in Western
- Not enough protein
- western blot stopping stages
- transfer issue, blotchy membrane
- laemmli buffer dilution
- Problem in Western blot
- denaturation of the protein
- western blot and phosphorylation
- Western Blot and Phosphorylation
- transfer buffer pH lower than 8.3
- ponceau washed away with dH2O?
- Mystery in Western
- dot blot and western blot
- E. coli and S. aureus Western Blot
- supernatant load control problems
- Please!!! too viscous samples!! I need help urgent!
- How long can you store a blotted membrane before you do a blot?
- internal control of western blot for E. coli
- a different way to develop
- Transfer problem
- antibody works in IHC but not on blot?
- anti-GFP antibody
- Small protein - washed away ?
- Western Blotting for Phosphorylated Proteins
- Positive Dot-Blot, negative WB
- Positive dot blot, negative WB
- Weird western
- blotchy, irregular bands
- Gel breakage during transfering on electroblotter! HELP PLEASE
- alternate way for quantification ?
- weak signal! ! ! !
- Western blotting allergen proteins for human IgE specificity
- Bands in the wrong place
- Membrane Storing
- Wierd blot behavior
- Collagen IV and TGF beta help
- Do You Use ImageJ in Densitometry?
- Western too many non specific bands
- low and weak bands
- stacking gel %
- Tris in Laemmli buffer
- nice tight and dark non specific band
- smaller size of eGFP fusion protein
- AP vs. HRP secondary
- Cutting down on the background
- Problem with Western Blot Transfer Conditions?
- pvdf membrane
- weird bands visible on blot after ECL
- blotting: room temperature vs cold room
- best lysis buffer to use
- Syngene GeneTools
- backgound issues in farwestern employing avidin-biotin
- Antybodies for caspases??
- Native proteins from isoelectric focusing gel
- protein cleavage during protein purification?
- No band
- Problems with GLUR1 antibody-HELP!
- Best loading control for plasma membrane fraction samples
- Why the band at wrong molecular weight?
- why white bands instead colored one in WB?
- Dot instead of a band on precast gel (running funny!)
- applying serum control bands
- practical experience with paper based peptide enzyme immunossay???
- whole cell lysate
- Keep antibody mix at -20
- refrigerator malfunction - frozen membranes
- Lysing with higher concentration of SDS for WB?
- Gel Stick
- home made ECL
- Why lyse on a plate and scrape?
- Biotinylated peptide marker???
- immunoglobulin depletion
- Western Blot Membrane Stripping Reprobing Disadvantages
- Western Blot Stripping Blots
- problem with tubulin bands
- Mysteriously light S6 bands
- Western blot secreted protein control
- Western Blot Mystery - Developed bands don't adhere to membrane
- equal loading on western blot?
- Loading control for serum samples?
- yellow-green line on sds gel
- Unidentified problem with low concentrated samples
- Protein not migrating down gel
- Western Blot
- double bands in western blot
- Smeared bands on only half of the blot
- Nuclear proteins
- Does anyone know runx2 antibody?
- transfer problem
- nuclear extraction buffer!
- gel grew during transfer
- detection of phosphorylation in western blot
- How to fix the problem?
- Gel with double interface?
- Whatman filter paper
- Loading control for membrane fractions
- Can we use dot blot inplace of Western blot?
- Smeary western blot
- Inverted Blot
- SDS-PAGE Voltage not Ramping Up
- band of interest is too low
- transfer in western blot
- phospho gsk3beta!!!!
- Tertiary detection system for western blotting
- EtBr in Western?
- BLOTS are UUUUGLLLLY...:(
- Recombinant positive control dose?
- Transfer Issues to PVDF
- unspecific bands all over wb
- Way too many bands on western blot
- background troubleshootings supersignal femto
- Cytochrome C Detection Western Blotting
- Mouse Brain Western Blot Detection
- primary vs secondary antibody
- SDS Vs Tween
- Tbst
- pH of transfer buffer
- can I detect inclusion bodies on westernblots???
- Immunohistochemsitry Quantification
- Western blotting quantification
- Antibody Reuse for Western Blots?
- Non-Denaturing Western Blot Transfer?
- Cloudy Western Blot
- Problem with signal detection
- can gel be stored before transfer?
- Can't get loading controlto work after stripping
- Antibody dilution
- TBS using Tris base vs Tris HCl
- Blocking for 1h vs overnight
- Please clarify me regarding loading standard.
- can stripping buffer be re-used?
- Does anyone know of newer methods that are better at detecting protein expression...
- Reusing Antibody After Western Blot
- Ubiquitinated Protein
- two primary antibody
- Western Blot Vs Immunohistochemistry
- transfer time
- two bands
- GAPDH Not visible on western blot
- Western Blot Problem
- Optimal protocols for restripping membranes
- cross reaction of 1st antibody
- crude plasma membrane
- Western Blot against Caspases
- negative band on film
- Sample Buffer
- Anyone treating Cell lysates with RNAse/DNAse
- p-ERK..
- PBS in fractions
- Loading control?
- HRP marker
- Multiple Antibodies at once?
- Where is the protein ladder?
- internal control for plant proteins in WB
- Staining the gel and imaging
- c kit WB
- Increase Exposure, Bands Disappear??
- 1 gel, 2 probes
- Phospho proteins
- Fast Westerns
- recombinant protein has a bigger MW than control which is the same protein
- Recommended Western Blot Membrane Supplier
- plasma membrane fraction
- Difference between PBS/TBS and Tween/TritonX?
- Help Migrating bands
- white sample band but black positive control band
- Question on cold pack
- Western problems
- Transglutaminase
- GADPH Loading Control Western Blot Problem
- Voltage_question
- Blotchy/White Spotted Membranes
- Internal controls for WB
- Non-specific antibody.
- Collagen type 1 expression, help needed
- Blotting a nuclear protein and nuclear protein extractions in tissue
- Linear vs conformational eptiopes on Western Blot
- Absence of Bands on Western Blot Film
- unblocking a membrane
- western blotting
- WB anti-caveoline1 not reproducible
- Western Blotting reagents
- Protein Denaturation
- IP washing step
- Western Blot Membrane Incubation with NP-40?
- Western Blot Background Troubleshooting
- quantify disturbed gel
- Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please
- Storage of PVDF membranes after western
- Dot blot: dots on film bright white/clear not black?
- Novel(?) Western Blotting question
- Film Black Western Blot Glowing Dark
- air bubble while loading sample.
- No Bands on Western Blot
- How to Store Western Blot Membranes Later Use
- Western Blot Info
- transfection
- Bcl-2 is too weak
- Loading Control
- Electrophoresis problem
- Invitrogen and stripping
- Filter paper for western blotting
- Convex shape of sample bands on SDS PAGE gel
- brain tissue vs other tissues
- 11KD detection
- Electrophoresis Problems
- western blot quantitation
- Stripping Blots Protocols
- Western Blot Woes - Very Clean Western
- Western blooot troubleshooting
- Western Blot Densitometry
- Western Blot - Extra Bands
- Membrane Stripping and Number of Times you can Blot
- Western Blot Protocol
- Western Blot Info
- Blocking Membranes for Western Blotting
- Western Blot Optimization