- type of collagenase for chondrocytes isolation
- Non-specific band present after immunoprecipitation
- EMSA two fragment bands
- autofluorescence
- Mold problem in 4°C room
- Mold problem in 4°C room
- Mold problem in 4°C room
- Mold problem in 4°C room
- Mold problem in 4°C room
- Mold problem in 4°C room
- S2 Schneider Huge growth
- (no subject)
- tm calculation for tagged primers
- tm calculation for tagged primers
- Low melting point agarose
- How can I dissolve it ????
- something about AflII
- adding kozak sequence to primer
- Inclusion bodies centrifugation
- Inclusion bodies centrifugation
- Custom ISH probe synthesis
- adding kozak sequence to primer
- MEG vs. PEG
- MEG vs. PEG
- Loading dye
- Loading dye
- PCR Lab Design
- International qPCR 2010 Symposium & Exhibition in Vienna
- anti Tag antibody cross reactivity
- chloroform
- chloroform
- JM101 for Protein Expression?
- JM101 for Protein Expression?
- JM101 for Protein Expression?
- PCR Lab Design
- insitue PCR
- Lentiviral Vector
- Peptide/Amino Fusion by Chemistry
- (kein Betreff)
- (kein Betreff)
- (kein Betreff)
- (kein Betreff)
- Re:Re:Re:PhiX174 resistance E. coli strain?
- Re: PhiX174 resistance E. coli strain?
- Free siRNA Design software
- Maximun size of DNA obtained thru miniprep matrix
- Maximun size of DNA obtained thru miniprep matrix
- Maximun DNA size in miniprep
- rna storage at -20
- Re: hi
- IP Question
- rna storage at -20
- 3' end labelling with tritiated thymidine...
- qRT-PCR on very few (10,000 - 20,000 cells)
- PhiX174 resistance E. coli strain?
- hi
- hi
- Percoll for PMN-Isolation
- Mitochondrial isolation for mt DNA extraction from Earthworms
- fusion protein construction
- fusion protein construction
- problem with competent cells
- Methods Digest, Vol 53, Issue 1
- Short Vector Multiple Cloning Sites/Polylinkers
- Passive Lysis Buffer for Promega Dual Luc assay
- mitochondrial DNA copy number measurement
- Reactome Pathway Database User Survey
- can T7/SP6 transcribe eukaryotic DNA without bias
- qPCR NEWS - September 2009 - key topic - circulating nucleic acids -CNA
- Problems with Establishing Standard Curve in ELISA
- Reusing 96 well flourescence plates
- LPS purfication
- clean-up of ssDNA
- Cot1 DNA preparation
- RNA dot blot protocol?
- qRT-PCR on very few (10,000 - 20,000 cells)
- qRT-PCR on very few (10,000 - 20,000 cells)
- Primer designing for expression vector
- Primer designing for expression vector
- pTopo purification problems
- Help in calculatiing Tm and GC content of primer
- Methods Digest, Vol 52, Issue 7
- pTopo purification problems
- pTopo purification problems
- pTopo purification problems
- pTopo purification problems
- RNAseH
- RNAseH
- RNAseH
- RNAseH
- cellular localization of L-arginine on confocal
- protein purification problem
- protein purification problem
- electroporation
- Total RNA extraction from whole blood
- Conditions for use, Tet-On system
- SDS concentration measurement
- Semi sticky surface for shaking platform
- Semi sticky surface for shaking platform
- Semi sticky surface for shaking platform
- DNA isolation from hair
- low LPS plasmid prep protocol
- Redissolving lyophilised proteins?
- copy number determination real time vs sauthern blott
- copy number determination real time vs sauthern blott
- Problem in Bradford assay
- miRNA target
- Silver/gold staining and PCR amplification
- seap reporter vector
- AFLP smear
- pfu pc
- malonyl-coa
- qPCR NEWS August 2009 - focus on HRM
- PCR for DNa walking
- Methods Digest, Vol 51, Issue 9
- pfu pcr
- pfu pcr
- pfu pcr
- pfu pcr
- fusion protein purification problems
- I am looking....
- Methods Digest, Vol 51, Issue 4-VECTORS SEQUENCES
- Zinc or Iron binding protein, that is a question!
- VECTORS SEQUENCES
- fusion protein purification problem
- VECTORS SEQUENCES
- An effective method for elimination of DNA and RNA residues
- Ab smear obscures signal on IP-western
- Eiken agar/media
- Seeking method for fatty acid content determination in plant seedsand seedlings
- reconstitution of membrane proteins
- phmeter
- Msp A1I restriction enzyme
- About SSCP
- About SSCP
- guanidinium disposal
- storing of enzyme
- AGROBACTERIUM
- Endotoxins
- qPCR NEWS July 2009 - focus on single-cell qPCR
- Confused about the different principles to freeze & thaw cellsand purified protein
- Fwd: Confused about the different principles to freeze & thaw cellsand purified protein
- Confused about the different principles to freeze & thaw cellsand purified protein
- Confused about the different principles to freeze & thaw cellsand purified protein
- Confused about the different principles to freeze & thaw cells andpurified protein
- Standard Curve qPCR
- E. coli mutator strain
- can protein modifications affect immunodetection?
- Cultures
- [a bit OT] floating "precipitates"
- Homemade chemifluorescence
- TEV
- Kind Attent
- Help in Primer designing for expression...
- protein extraction
- Help in Primer designing for expression...
- leptomycin B
- DMSO Alternative?
- cleaning coverslip for protein/cell attachment
- (no subject)
- Biotin contamination of BSA
- cleaning coverslip for protein/cell attachment
- Aggregation protein purification problem, help.
- flurescent proteins
- flurescent proteins
- invitrogen flp-in
- Glycogen Assay help
- Sybe Safe as a cell marker
- luciferase reporter assay
- strand displacement activity assay
- YNB plates not setting
- Agar Plate Question
- please help
- please help
- please help
- agarose electrophoresis tank @ $10
- agarose electrophoresis tank @ $10 (aria johnson)
- agarose electrophoresis tank @ $10
- agarose electrophoresis tank @ $10
- agarose electrophoresis tank @ $10
- qPCR NEWS June 2009 - MIQE media & press review
- Methods Digest, Vol 49, Issue 18
- Puzzling ICP-ES Results, Metal Binding Protein?
- help in PCR
- BigDye Sequencing with DMSO
- help in PCR
- Lectins
- composition of water and detergent
- How is size of Tap-Tag, protein A, and Tev site, calmoduline binding protein
- How to dissolve blood clots formed in EDTA tubes?
- How to exchange antibiotic resistance markers on plasmids for E.coli
- problems with deoxycholate precipitation
- How to exchange antibiotic resistance markers on plasmids for E.coli
- AT&T Usenet Netnews Service Shutting Down
- How to exchange antibiotic resistance markers on plasmids for E coli
- Methods Digest, Vol 49, Issue 12
- Methods Digest, Vol 49, Issue 12
- Western Blotting problems (Alsagaby, Suliman)
- How to prepare this solution??
- ER Special Dyes
- Total Protein (BioRad)
- Total Protein
- Methods Digest, Vol 49, Issue 4
- oligo and generunner manual
- Promoter amplification
- Cytoxicity dose range is too narrow
- How to effectively prevent protein aggregation?
- RNA isolation from pigeonpea
- EDTA solution becomes slurry
- no antibiotics when transfecting/infecting. Why?
- Restriction Enzyme Help: Sac II
- expression of a subunit
- Using Histidine as a Nickel Elution Buffer
- Benzoic Acid Assay
- EDTA solution becomes slurry
- qPCR NEWS May 2009 - focus on RNA integrity
- expression of a subunit
- Help with PCR reaction Phusion Flash - wrong sized fragment
- Help with PCR reaction Phusion Flash - wrong sized fragment
- molecular biology textbooks
- cho DHFR
- 15% native gel shift expt.
- 15% native gel shift expt.
- 15% native gel shift expt.
- Last Announcement: EMBO Conference Series on Protein Synthesis andTranslational Control
- RNA isolation failed
- Glyco stain
- 15% native gel
- SUMO antibody problem (Nikola Wenta)
- RNA based FISH on frozen tissue samples
- RNA labeling at 5'
- RNA labeling at 5'
- Monomeric Abeta detection in brain of APP tg mice using westernblotting
- protein extraction
- Monomeric Abeta detection in brain of APP tg mice using westernblotting
- Acid phosphatase
- Crossing in Arabidopsis
- molecular biology textbooks
- Methods Digest, Vol 48, Issue 10
- Hello - Thrombin Cleavage of GST
- self- digesting dna problems
- pET23d vector
- Real time pcr multiplex with probe vs. sybrgreen
- MMTV as a reponsive element is a lucifierase reporter construct
- proteomics problem
- smart oligo
- problems with restriction digestion
- smart oligo
- Another source for Invitrogen YO-PRO-1
- embryo media