I am fine mapping a suppressor gene and after initial mapping I landed on Ch4 between c1w6 (10% recombination frequency (RF)) and g4539 (39% RF) marker. For fine mapping I need sequence information of Ws between these location. It would be a great help if someone out there can help me find any information on how to narrow-down and If there is any sequence info available for WS ecotype in this region.I used At Primer that generates CAPS primers for Co and Ws but did not see the predicted polymorphism between the two accessions. Please I am stuck at this point if some one can help I will greatly acknowledged there advice in my PhDwork. You can e-mail me at [Only registered users see links. ].