I am experiencing a weird trait in my WT columbia Arabidopsis where their roots are for the most part all growing away from the media.
I am just doing a simple experiment in seeing which conditions grow better for the lab in varying MS and sucrose levels.
each media contains:
.5-1x MS pH 5.7
1 g/L MES buffer
and they were plated in normal circular petridishes placed in a cold 4 deg room for 3 days before I put them in a plant growth room laid flat.
I saw this weird root growth trait (where roots should be growing either into the agar or at least on top, if agar is too hard) about 7 days into development and 3 weeks later it is more so where they are growing upwards all over the place on top of the agar.