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autoclaving kinetin and NAA

autoclaving kinetin and NAA - Arabidopsis and Plant Biology

autoclaving kinetin and NAA - Discuss Arabidopsis and Plant Biology Research.


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Old 07-22-2010, 03:38 PM
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Default autoclaving kinetin and NAA



Hello everyone,

I am currently using kinetin and NAA from Sigma and their product support said it is best not to autoclave these with my media. I've been adding them after my autoclaved media has cooled in a hood. This works fine for pouring plates but I'd like to be able to distribute my media into tubes and autoclave the tubes. I was wondering if anyone has experience autoclaving these hormones?

Thanks in advance
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Old 07-28-2010, 06:43 AM
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Default Re: autoclaving kinetin and NAA

Hi tecnikal,

why u want to autoclave this hormone?........well if u want, then check the optimum temperature for NAA in any biochemistry book firstly then go ahead bcz hormones r basically proteins and any protein can be stable at a definite temp. only.....so be sure that ur NAA will not be denatured upto which temp.......otherwise u would be loosing the results...

goodluck for ur experiments.
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