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Endogenous Biotin in Arabidopsis?

Endogenous Biotin in Arabidopsis? - Arabidopsis and Plant Biology

Endogenous Biotin in Arabidopsis? - Discuss Arabidopsis and Plant Biology Research.

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Old 05-26-2010, 02:28 PM
Pipette Filler
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Default Endogenous Biotin in Arabidopsis?

Hey out there Arabidopsis folk,

Quick question for you all. I have read snippets suggesting that Arabidopsis contains endogenous biotin. So my question is whether this poses a problem for applications like ELISA in which I am using Streptavidin labelled with Alkaline Phosphatase to bind an antibody labelled with biotin?

Could I be getting high background in protein extracts because Streptavidin-AP is binding to Arabidopsis' native biotin molecules?

If so, what can I do about this?

Thanks in advance,

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alkaline phosphatase , arabidopsis , biotin , endogenous , streptavidin

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