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FLX shotgun sequencing -help please!

FLX shotgun sequencing -help please! - Arabidopsis and Plant Biology

FLX shotgun sequencing -help please! - Discuss Arabidopsis and Plant Biology Research.


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Old 12-16-2009, 01:40 PM
Pipette Filler
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Default FLX shotgun sequencing -help please!



Hi everbody!

I urgently need help with FLX 454 sequencing, I'm totally new in this area. So my questions could be so basic. I have a plant with no genomic sequence information available and want to get some random sequences from this plant which I will later try to detect ssr's (or microsatellites) with use of some bioinformatic programs. I don't have gross budget to use the whole genome sequencing function of the FLX.

I thought about using the shotgun sequencing system of FLX but I don't know how. Should I first digest my gDNA's and run them on a gel and than just extract some size of interest and enrich these fragments with cloning in bac or some vectors? Could anyone help me? Please, help me,
Thanks a lot
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