I urgently need help with FLX 454 sequencing, I'm totally new in this area. So my questions could be so basic. I have a plant with no genomic sequence information available and want to get some random sequences from this plant which I will later try to detect ssr's (or microsatellites) with use of some bioinformatic programs. I don't have gross budget to use the whole genome sequencing function of the FLX.
I thought about using the shotgun sequencing system of FLX but I don't know how. Should I first digest my gDNA's and run them on a gel and than just extract some size of interest and enrich these fragments with cloning in bac or some vectors? Could anyone help me? Please, help me,
Thanks a lot