Removal of the lower epidermal layer from mature rosette leaves?
Dear Arabidopsis community,
We are looking for a method to conveniently remove the lower epidermal layer (and preferably mesophyll) from mature rosette leaves. This is for a high throughput screen so the procedure needs to be relatively simple. We have tried floating leaves o/n on pectinase or physically peel away the epidermis, but the latter is way too tedious for the numbers of leaves we need to process. Scraping on fine sandpaper has not worked for us either. If anyone has ideas or proven methods we would be glad to hear.
Re: Removal of the lower epidermal layer from mature rosette leaves?
What is the downstream application intended for these cells?
|All times are GMT. The time now is 06:37 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved