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[Arabidopsis] GUS staining in Alfalfa

[Arabidopsis] GUS staining in Alfalfa - Arabidopsis and Plant Biology

[Arabidopsis] GUS staining in Alfalfa - Discuss Arabidopsis and Plant Biology Research.


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Old 10-08-2009, 03:17 PM
JP Fonseca
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Default [Arabidopsis] GUS staining in Alfalfa



Hi Lisa,

Not very familiar with Alfafa. I have observed GUS staining in Arabidopsis
before by Agroinfiltrating leaves with a needleless syringe containing my
GUS reporter constructs. Then allow transformed samples to rest for ~ 2 days
to allow GUS expression and then incubate samples in GUS staining solution
overnight at 37 C.
Next day washed several times with ethanol 70% to remove clorophyll and...
"voila!"...blue GUS spots observed.
Hope it works in Alfafa.

JP Fonseca





On Wed, Oct 7, 2009 at 5:12 PM, LISA M KOCH <[Only registered users see links. ]> wrote:

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alfalfa , arabidopsis , gus , staining


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