Not very familiar with Alfafa. I have observed GUS staining in Arabidopsis
before by Agroinfiltrating leaves with a needleless syringe containing my
GUS reporter constructs. Then allow transformed samples to rest for ~ 2 days
to allow GUS expression and then incubate samples in GUS staining solution
overnight at 37 C.
Next day washed several times with ethanol 70% to remove clorophyll and...
"voila!"...blue GUS spots observed.
Hope it works in Alfafa.
On Wed, Oct 7, 2009 at 5:12 PM, LISA M KOCH <[Only registered users see links. ]> wrote: