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| Dear all, I have two T-DAN insertion Arabidopsis lines. The two knock-out loci are about 1Mb in distance on chromosome V. After genetic cross of the two single mutants, F1 plants were self-pollinated and seeds were harvested. My question is how many F2 individuals I would need to ensure one expected double mutant. Thanks a lot for your help. Yin |
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| inheritance , linkage |
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