linkage inheritance
 

Dear all,

I have two T-DAN insertion Arabidopsis lines. The two knock-out loci are about 1Mb in distance on chromosome V.
After genetic cross of the two single mutants, F1 plants were self-pollinated and seeds were harvested.
My question is how many F2 individuals I would need to ensure one expected double mutant.
Thanks a lot for your help.

Yin