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Arab-gen Digest, Vol 51, Issue 7

Arab-gen Digest, Vol 51, Issue 7 - Arabidopsis and Plant Biology

Arab-gen Digest, Vol 51, Issue 7 - Discuss Arabidopsis and Plant Biology Research.

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Old 07-15-2009, 10:47 PM
Cesar Barragan
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Default Arab-gen Digest, Vol 51, Issue 7

Hello Abo-Ogiala

Another source you might want to check is our website (http://
signal.salk.edu), here you can find more information about these
lines or any other you may be interested. As an example, you will
find that line SALK_146631 is a contaminated line so you could try
instead SALK_139052 or SALK_127904 ([Only registered users see links. ]
tdnaexpress). Information about this problem can be found in the FAQ
section ([Only registered users see links. ]) as follow:

16. Q: On the T-DNA Express website, some insertion lines are very
close in number or have the same number. For example SALK_005299,
005300, 005301, and 005305) have the same sequence. How can this be?

A: We have found that the ABI 384-well sequence precipitation
method can lead to cross-contamination of samples. A new protocol was
devised to reduce the number of contaminating sequences. When
verifying insertions of interest you should order and test all lines
listed for that gene. The most definitive test to determining if the
line contains the expected gene insertion is to use Southern
blotting. Often times PCR can give a false negative result. We
strongly suggest that you carry out Southern blotting and
hybridization with the labeled gene as a probe to determine if the
line you received contains an insertion in the expected gene.

We hope these information and links can be useful for you and the
Best Wishes.

Cesar Barragan
The Salk Institute for Biological Studies
Genomic Analysis Laboratory
10010 North Torrey Pines Road
La Jolla, CA 92037
Tel 858-453-4100 ext 1796
[Only registered users see links. ]

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