Arab-gen Digest, Vol 50, Issue 15

Dear Chuanmei Zhu
DBBS(plant biology),
Washington University, St.Louis, MO,USA. 63130

the genomic DNA of arabidopsis has the introns,UTRS,etc thats why you may
not be able to amplify the expected amplicon, so its better to amplify from
CDNA, if you have plant tissue prepare RNA , from that prepare CDNA using
superscript or effecient MMLV(RTenzyme). so that you may amplify exact
amplicon then try to clone to cloning vector and subsequently sequencing and
binary vector or interaction studies etc . hope this may give you some idea..
take care




On 6/30/09, [Only registered users see links. ] <
[Only registered users see links. ]> wrote:



--
Ramu.S.V
Senior research fellow
dept of crop physiology,
Molecular plant physiology lab,
UAS, GKVK, Bangalore