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[Arabidopsis] request for info on T-DNA orientation

[Arabidopsis] request for info on T-DNA orientation - Arabidopsis and Plant Biology

[Arabidopsis] request for info on T-DNA orientation - Discuss Arabidopsis and Plant Biology Research.


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  #1  
Old 06-15-2009, 11:40 AM
Abo-Ogiala, Atef
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Default [Arabidopsis] request for info on T-DNA orientation



Please I need answer for the same question:

I want to find the orientation of T-DNA insertion in some mutants of
Arabidopsis.



Best regards

Atef Abo-Ogiala

PhD student

Büsgen-Institut

Forstbotanik und Baumphysiologie

Georg-August Universität

Büsgenweg 2

37077 Goettingen

Germany



Tel: +49 (0)551 - 39 9362

Fax: +49 (0)551 - 39 22705



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Old 06-17-2009, 08:15 PM
Adrian
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Default request for info on T-DNA orientation

On Jun 15, 7:40*am, "Abo-Ogiala, Atef" <[Only registered users see links. ]-
goettingen.de> wrote:

Depending from which mutant bank you request the mutants is possible
to see the orientation of the insertion on the SIGnAL T-DNA Express
tool ([Only registered users see links. ]), just need the AGI
number of the interrupted gene and the code of the mutant. The page
will display the gene orientation on the genome and the orientation of
all T-DNA insertion mapped to that gene. If you have your own mutant
bank generated by T-DNA insertion, to find the T-DNA orientation you
should perform a TAIL-PCR using the T-DNA that you prefer.

I hope this help...

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arabidopsis , info , orientation , request , tdna


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