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Arabidopsis roots growing on top of phytagel in plates

Arabidopsis roots growing on top of phytagel in plates - Arabidopsis and Plant Biology

Arabidopsis roots growing on top of phytagel in plates - Discuss Arabidopsis and Plant Biology Research.


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  #1  
Old 06-08-2009, 07:20 PM
Pipette Filler
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Default Arabidopsis roots growing on top of phytagel in plates



Hey all! I am running into some inconsistencies when growing arabi on gel plates. I use a 2% phytagel in square plates, start them off flat for 3d then tip at 45deg angle so that the roots grown down against the bottom of the plate, UNDER the gel. I'm running into quite a few plants that show roots growing on TOP of the gel, instead of burrowing down underneath... About 1/2 of my plants end up with the root system on top of the gel instead of under the gel against the inside wall of the plate. Anyone else have this issue?
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Old 06-09-2009, 03:16 PM
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Default Re: Arabidopsis roots growing on top of phytagel in plates

I just kept them flat, no tilting and never saw this problem.
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Old 09-01-2009, 07:30 PM
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Default Re: Arabidopsis roots growing on top of phytagel in plates

danfive, interesting, I've tried flat as well and they very often seem to grow on top of the gel instead of underneath. I wonder if my gel is too hard...
thanks!
a
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