Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Arabidopsis and Plant Biology
Register Search Today's Posts Mark Forums Read

Arabidopsis and Plant Biology Discuss Arabidopsis and Plant Biology Research.

PEG8000 use for water deficit

PEG8000 use for water deficit - Arabidopsis and Plant Biology

PEG8000 use for water deficit - Discuss Arabidopsis and Plant Biology Research.

LinkBack Thread Tools Display Modes
Old 05-25-2009, 07:04 AM
Posts: n/a
Default PEG8000 use for water deficit

Hi colleagues,
I want to use PEG8000 to introduce water deficit stress to Arabidopsis roots. What is the suitable concentration of it to induce mild dehydration stress, eg. in 1/2x MS medium? I do not like high concentration as it may causeplasmolysis (I personally do not like 200 or 300 mM NaCl used by so many Arabidopsis researchers in salt stress study). However, a too low concentration of PEG8000 may have slow effect in a short time. Is there anyone knowing a reasonable concentration of it in hydroponics system of Arabidopsis? I want to harvest the tissue in a few hours (less then half a day). How about10% or 15%?* Low concentration stimulates root elongation while high concentration won't, according to some references.
Thanks for your input.

Reply With Quote

deficit , peg8000 , water

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

All times are GMT. The time now is 03:36 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10811 seconds with 15 queries