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#1
04-13-2009, 09:39 PM
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 BASTA resistance gene silencing Dear Colleagues, Does anybody experienced BASTA resistance gene silencing? We are screening a large collection of T-DNA knockouts (mainly SAIL and SM lines), and for some lines no resistant seedlings were rescued, even after sowing 70 seeds (all germinated, the phenotype is compatible with BASTA sensitive seedlings). This could be produced by a lethal mutation that segregates in a distorted way, but we are wondering if anybody has experienced BASTA gene silencing, as described for kanamycing in some Salk lines. Thank you, Gabriel __ Dr. Gabriel León Profesor Asistente Centro de Biotecnología Vegetal Universidad Andrés Bello República 217, 1 piso Santiago - CHILE Tel: +56-2-7703035 Fax: +56-2-6615832 [Only registered users see links. ]  |
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| basta , gene , resistance , silencing |
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