kanamycin and landsberg erecta

[tibeu]

Dear all,

I have a T-DNA insertion line in the ecotype Landsberg erecta. I seem unable to select these plants on kanamycin though.
All of my wild type Ler continue to grow on kanamycin, while Col0 plants, which I put on the same plate as a test, are staying small and are clearly sensitive.
I am using MS medium with 0.75% plantagar. The problem is not the kanamycin or the plates (since Col0 are sensitive).
All of my transgenic plants are also staying alive, while I didn't expect all of them to be homo- and heterozygous for the insert.
Has anybody had a similar experience with the Landsberg erecta ecotype (all Kana-segregation is going fine with Col0 ecotype)

Kind regards,

Tine

[yourlabdata]

I dont want to be rude but have you tried different Ler lines... is it possible that your Ler line is contanimated with Kan res. seeds? a misslabeled seed packet maybe? happens to us on a regular basis... 1 drop in in a previous generation can cause havok in latter generations.