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avoiding sample movement in confocal microscope

avoiding sample movement in confocal microscope - Arabidopsis and Plant Biology

avoiding sample movement in confocal microscope - Discuss Arabidopsis and Plant Biology Research.


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  #1  
Old 02-06-2009, 09:11 AM
Damiani
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Default avoiding sample movement in confocal microscope



Dear members,

Using confocal microscopy I want to see fluorescence appear after
treatment of roots with various solutions.
The problem is that the treatment makes the sample move on the slide.
Thus, I cannot follow the fluorescence on the same focal plan.

Is anybody know how to fix a sample on a slide to avoid all the
movements of the sample?

Thank you.

Isabelle

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  #2  
Old 02-09-2009, 09:24 AM
Pipette Filler
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Default Re: avoiding sample movement in confocal microscope

Hi!!!
Can you please make your question bit clear? I mean what kind of seedlings you have are they GFP fusions or you are using/performing antibody(Flourscent-Conj.) treatments which is a kind of Wholemounts??

Cheers,
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