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avoiding sample movement in confocal microscope
Using confocal microscopy I want to see fluorescence appear after
treatment of roots with various solutions.
The problem is that the treatment makes the sample move on the slide.
Thus, I cannot follow the fluorescence on the same focal plan.
Is anybody know how to fix a sample on a slide to avoid all the
movements of the sample?
Re: avoiding sample movement in confocal microscope
Can you please make your question bit clear? I mean what kind of seedlings you have are they GFP fusions or you are using/performing antibody(Flourscent-Conj.) treatments which is a kind of Wholemounts??
|avoiding , confocal , microscope , movement , sample|
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