avoiding sample movement in confocal microscope

Dear members,

Using confocal microscopy I want to see fluorescence appear after
treatment of roots with various solutions.
The problem is that the treatment makes the sample move on the slide.
Thus, I cannot follow the fluorescence on the same focal plan.

Is anybody know how to fix a sample on a slide to avoid all the
movements of the sample?

Thank you.

Isabelle

[Plantplanet]

Hi!!!
Can you please make your question bit clear? I mean what kind of seedlings you have are they GFP fusions or you are using/performing antibody(Flourscent-Conj.) treatments which is a kind of Wholemounts??

Cheers,