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-   -   RNA extraction from dry seed (http://www.molecularstation.com/forum/arabidopsis-plant-biology/56274-rna-extraction-dry-seed.html)

Edwin Reidel 02-04-2009 06:33 PM

RNA extraction from dry seed
 
It's hard to beat the modified hot borate buffer developed for RNA
extraction from cotton seeds by Wan & Wilkins: Analytical Biochemistry:
223(1): 7-12.

Also check the citing papers for further modifications significantly
simplifying their protocol, especially Wu et al. (Plant Molecular Biology
Reporter. 20(3): 213-218) I routinely extracted intact, high-purity RNA
from various plant tissues with the hot borate buffer.

Regards,
Edwin J. Reidel
Scientist, Seed Production Research
Nunhems USA, Inc.

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