![]() |
RNA extraction from dry seed It's hard to beat the modified hot borate buffer developed for RNA extraction from cotton seeds by Wan & Wilkins: Analytical Biochemistry: 223(1): 7-12. Also check the citing papers for further modifications significantly simplifying their protocol, especially Wu et al. (Plant Molecular Biology Reporter. 20(3): 213-218) I routinely extracted intact, high-purity RNA from various plant tissues with the hot borate buffer. Regards, Edwin J. Reidel Scientist, Seed Production Research Nunhems USA, Inc. __________________________________________________ _____________________________________________ The information contained in this e-mail is for the exclusive use of the intended recipient(s) and may be confidential, proprietary, and/or legally privileged. Inadvertent disclosure of this message does not constitute a waiver of any privilege. If you receive this message in error, please do not directly or indirectly use, print, copy, forward, or disclose any part of this message. Please also delete this e-mail and all copies and notify the sender. Thank you. For alternate languages please go to [Only registered and activated users can see links. Click Here To Register...] __________________________________________________ _____________________________________________ |
| All times are GMT. The time now is 03:58 PM. |
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2013, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved