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[Arabidopsis] RNA extraction from dry seed

[Arabidopsis] RNA extraction from dry seed - Arabidopsis and Plant Biology

[Arabidopsis] RNA extraction from dry seed - Discuss Arabidopsis and Plant Biology Research.


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Old 02-04-2009, 12:50 AM
yuanqing@ualberta.ca
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Default [Arabidopsis] RNA extraction from dry seed



Hi,

Most kits do not work with dry seeds, in my view.
I ever extracted total RNA from dry At seeds using the following
protocol and got RNA comparable to young tissue RNA extracted using
Qiagen Plant RNeasy mini kit. Good luck!

Suzuki Y, Kawazu T, Koyama H (2004) RNA isolation from siliques,
dry seeds, and other tissues of Arabidopsis thaliana. Biotechniques
37:542544


Quoting "Zacharie LeBlanc" <[Only registered users see links. ]>:



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