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RNA extraction from dry seed

RNA extraction from dry seed - Arabidopsis and Plant Biology

RNA extraction from dry seed - Discuss Arabidopsis and Plant Biology Research.


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Old 02-03-2009, 11:47 PM
Zacharie LeBlanc
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Default RNA extraction from dry seed



Hi all, I'm trying to extract RNA from dry Arabidopsis seeds in order to do
some expression profiling. I'm currently using a homogenizer in order to
grind 0.1 grams of seeds then use the protocol given along with Plant RNA
Reagent by Invitrogen. verification of my product on a 0.1% agarose gel
hasn't yielded any positive results yet. any thoughts?
-Zach LeBlanc
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