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#1
12-17-2008, 03:36 PM
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 Immunostaining of live protoplasts Hi Arabineters. I want to find out whether a protein is surface exposed(plasma membrane bound) or intracellularly expressed in BY2 protoplasts.If I go for immunostaining,can anyone please suggest any fixing protocol that don't permeabilize protoplasts?Alternatively if I want to go for staining of live protoplasts,can anyone suggest any useful tips. Regards & Thanks Anirban -- Anirban Baral JUNIOR SCHOLAR NATIONAL CENTRE FOR BIOLOGICAL SCIENCES TATA INSTITUTE OF FUNDAMENTAL RESEARCH UAS-GKVK CAMPUS,BELLARY ROAD BANGALORE-560065,INDIA  |
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| immunostaining , live , protoplasts |
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