Immunostaining of live protoplasts
I want to find out whether a protein is surface exposed(plasma membrane
bound) or intracellularly expressed in BY2 protoplasts.If I go for
immunostaining,can anyone please suggest any fixing protocol that don't
permeabilize protoplasts?Alternatively if I want to go for staining of
live protoplasts,can anyone suggest any useful tips.
Regards & Thanks
NATIONAL CENTRE FOR BIOLOGICAL SCIENCES
TATA INSTITUTE OF FUNDAMENTAL RESEARCH
UAS-GKVK CAMPUS,BELLARY ROAD
|All times are GMT. The time now is 03:44 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved