Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Arabidopsis and Plant Biology
Register Search Today's Posts Mark Forums Read

Arabidopsis and Plant Biology Discuss Arabidopsis and Plant Biology Research.


[Arabidopsis] brands of agar

[Arabidopsis] brands of agar - Arabidopsis and Plant Biology

[Arabidopsis] brands of agar - Discuss Arabidopsis and Plant Biology Research.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-11-2008, 10:04 AM
Forde, Brian
Guest
 
Posts: n/a
Default [Arabidopsis] brands of agar



Hi Tanya

For the last 15 years or so we have been routinely studying Arabidopsis
root growth and branching on vertical 'agar' plates, for up to two weeks
after germination. We have used 0.8% agar-agar in the past with
reasonable results, but found that batches were variable and sometimes
root growth would be inhibited. We now choose to use PhytagelTM, which
is purer and gives a clearer gel, and we have had no problems with
different batches (note that you have to make sure that the divalent
cation concentration in the medium is at least 2 mM, otherwise it won't
set!).

As an important aside, I would strongly recommend that you *don't* use
MS (or even 1/2 MS) for anything other than tissue culture or antibiotic
selection. It is an extremely rich medium with non-physiological N
concentrations that far exceed anything that plants are ever likely to
have experienced in nature (40 mM nitrate + 20 mM ammonium)and root
growth in this medium is also very slow. We use a dilute form of B5
medium that allows much faster root growth. We have done direct
comparisons with Ler and Col-0 growing in MS, 1/2 MS and in our modified
B5 medium at different rates of N supply and found that all growth
parameters (lateral roots, primary roots, shoots) are faster on the
modified B5 medium. The modified B5 medium also allows you to vary
individual components of the nutrient supply without creating severe
nutrient imbalances that cause plant stress. The composition of our
medium was originally published in Zhang & Forde (1998) Science 279,
407-409, but I can give you more details if you wish to email me.

Good luck!

Brian


=====================================
Brian G. Forde
Prof. of Environmental Plant Biotechnology
Department of Biological Sciences
Lancaster Environment Centre
Lancaster University
Bailrigg
Lancaster LA1 4YQ
tel. +44 (0)1524 510207 (direct line)
email [Only registered users see links. ]
[Only registered users see links. ]

Editor-in-Chief Plant Methods
email [Only registered users see links. ]
[Only registered users see links. ]
=====================================



-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of Falbel, Tanya
G.
Sent: 09 February 2008 21:11
To: [Only registered users see links. ]
Subject: [Arabidopsis] brands of agar


Colleagues:

Over the years, I've used several brands of agar for growing Arabidopsis
seedlings on MS medium, from Gibco, Sigma, and others. I've heard that
some groups use Noble agar, others, bacto-agar, others in the past have
washed their own agar, used phytagel or other
gelling agents. I have two questions:

1) What brands of agar are most commonly in use now by groups? I've
found something similar to Gibco's Phytagar that is now available
through Caisson labs. Other groups seem to like Sigma's A1296. But
besides those, what do most groups use? I'm especially interested in
the opinion of groups that measure root length or root branching -
growing roots vertically on plates for more than just a couple of days.
(as opposed to just antibiotic selection)

2) Does anyone know what inhibitors are washed away? Are micronutrient
ions or other toxic compounds bound to the crude agar? Has anyone
looked into this? This may have been a question that came up among
researchers 10 years ago, but I couldn't find any record in the
archives.

Here's why I'm asking. I made up a batch of medium with a brand of
'purified agar for microbial use', and got a very strong inhibition of
root meristems. Seed germination was good, but roots failed to elongate
any further after about 4 days of growth. The root meristems became a
swollen mess, reminiscent of what happens in weak gnom alleles. I'm
trying to decide if this is something worth looking into.

I know that for regeneration of plants in tissue culture, folks are very
fussy about particular brands of agar.

Please let me know what brands you have used with success or failure.
I'd be especially interested in other similar experiences - where a
brand wreaked havoc on root meristems.

Thanks

Tanya Falbel
Department of Biology
105 Garfield Avenue
University of Wisconsin-Eau Claire
Eau Claire, WI 54702-4004, USA

Tel: 715-836-5087
Fax: 715-836-5089


_______________________________________________
Arab-gen mailing list
[Only registered users see links. ] [Only registered users see links. ]

Reply With Quote
Reply

Tags
agar , arabidopsis , brands


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
[Arabidopsis] brands of agar Tobias Baskin Arabidopsis and Plant Biology 1 10-31-2010 08:40 PM
[Arabidopsis] brands of agar Forde, Brian Arabidopsis and Plant Biology 1 10-31-2010 08:39 PM
[Arabidopsis] brands of agar Smalle, Jan A Arabidopsis and Plant Biology 0 02-15-2008 03:58 PM
[Arabidopsis] brands of agar Grant Cramer Arabidopsis and Plant Biology 0 02-13-2008 07:15 PM
[Arabidopsis] brands of agar Tobias Baskin Arabidopsis and Plant Biology 0 02-11-2008 02:33 PM


All times are GMT. The time now is 10:14 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12656 seconds with 16 queries