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Postdoctoral position - 454 transcriptome sequencing of Kalanchoefedtschenkoi

Postdoctoral position - 454 transcriptome sequencing of Kalanchoefedtschenkoi - Arabidopsis and Plant Biology

Postdoctoral position - 454 transcriptome sequencing of Kalanchoefedtschenkoi - Discuss Arabidopsis and Plant Biology Research.


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Old 02-05-2008, 12:39 PM
James Hartwell
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Default Postdoctoral position - 454 transcriptome sequencing of Kalanchoefedtschenkoi




UNIVERSITY OF LIVERPOOL, UK

PLANT SCIENCES RESEARCH GROUP

SCHOOL OF BIOLOGICAL SCIENCES




You will join a 3-year BBSRC-funded project “Sequencing the
trancriptome of Kalanchoe fedtschenkoi: a model for Crassulacean acid
metabolism (CAM), embryogenic plantlet formation and the
Saxifragales”. This is a ground-breaking project that will employ the
latest ultra high-throughput pyrosequencing technology (Roche/454 GS
Leonardo) to achieve deep sequencing of the transcribed portion of
the K. fedtschenkoi genome.




POSTDOCTORAL RESEARCHER



£28,289 - £29,139 pa



You will generate cDNA samples for 454 sequencing and undertake
digital northern analysis of the 454 data to identify the genes
required for the circadian control of CAM, plantlet formation and
other unique aspects of K. fedtschenkoi's biology. You will
collaborate with a second bioinformatics postdoc to build contigs
from the 454 reads, and develop a K. fedtschenkoi EST database to
provide access to the ~15 billion base pairs of K. fedtschenkoi DNA
sequence that you will be involved in generating. For the CAM-
associated genes identified through the 454 sequencing, you will
perform detailed circadian transcriptome analysis using a Beckman-
Coulter GeXP system to profile the transcript abundance of groups of
30 genes simultaneously. CAM-associated regulatory genes will be
manipulated by generating transgenic K. fedtschenkoi lines, and you
will perform detailed phenotypic analysis to study the ability of
these lines to perform CAM. You will also coordinate the production
of a K. fedtschenkoi BAC library, clone BACs for CAM-associated
genes, and sequence the BACs using the Roche/454 GS Leonardo.



You should have (or be about to obtain) a PhD with experience in
plant molecular biology, biochemistry and/or physiology with
preference being given to individuals with experience of analysis of
large genomics data sets. Ideally, you will also have experience of
generating plant binary constructs for RNAi and overexpression, and
working with non-model plant species. Job Ref: R-567182


RESEARCH TECHNICIAN GRADE 5



£20,458 - £22,332 pa



You will be responsible for the growth and maintenance of K.
fedtschenkoi, isolation of RNA and DNA samples, generating transgenic
lines to manipulate key CAM genes, and the study of the phenotypes of
these lines.



You should be qualified to BTEC Higher level (or equivalent) with
experience in plant molecular biology, biochemistry and/or physiology
with preference being given to individuals with experience of tissue
culture based plant transformation. Ideally, you will also have
experience of PCR, RT-PCR, and enzyme and metabolite assays. Job Ref:
S-567180



Closing date for both posts: 22 February 2008


For informal enquiries, please contact Dr. James Hartwell, e-mail:
[Only registered users see links. ]

Weblink for Hartwell lab research interests: [Only registered users see links. ]
biolsci/people/academic/hartwell_j/index.html



For full details, or to request an application pack,

visit [Only registered users see links. ]

or e-mail [Only registered users see links. ]

Tel 0151 794 2210 (24 hr answerphone),

please quote Ref: in all enquiries

Dr. James Hartwell,
Lecturer in Plant Metabolism,
School of Biological Sciences,
University of Liverpool,
Crown Street,
Liverpool,
L69 7ZB
United Kingdom
Tel: +44 (0)151 795 4561
Fax: +44 (0)151 795 4410
E-mail: [Only registered users see links. ]
Web: [Only registered users see links. ]



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