Wheat Transformation

I am working on wheat (Triticum aestivum L.) transformation. I have made some transgenic plants, but unfortunately after regeneration from callus generated from immature embroys, they are dying in plastic pots.

In plastic pots, I have used soil+sand mix, soil+peat, vermiculite+peat seperatly in different experiments. but still the plants are not sustaining their growth.
their roots were also well grwon in MS medium.
the temeperature in that growth room is 24 degree celcius.
if any body has the knwoledge of wheat tissue culture plz, let me know.
thnanks,

Muhammad Shahzad Anjam

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Today's Topics:

1. Gentamycin selection ([Only registered users see links. ])


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Message: 1
Date: Tue, 21 Aug 2007 15:34:14 -0500
From: [Only registered users see links. ]
Subject: [Arabidopsis] Gentamycin selection
To: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ].edu >
Content-Type: text/plain; charset=ISO-8859-1; format="flowed"

Hi All

I am working on some Arabidopsis plants carrying the gentamycin marker.
For some reason, germination is bad on MS media. I would like to know
if anyone has selected for plants on soil by watering with gentamycin
mixed water or by applying a spray of gentamycin solution?

I really appreciate any suggestion in this regard.

Thanks
Savita



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End of Arab-gen Digest, Vol 28, Issue 14
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Muhammad Shahzad Anjam
Research Associate
Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE),
Faisalabad, Pakistan.
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Cell Phone: +92-333-8174001

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