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#1
07-17-2007, 09:05 AM
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 RNA extraction from etiolated seedlings We are in a process to built our own cDNA library and therefore we are trying to isolate RNA from etiolated seedlings. Up to now we tried column-based kits (Qiagen and Promega) and used TriFast solution to isolate RNA but did not receive suitable RNA. Now we would like to use a classical protocol using phenol and LiCl. We would be very thankful for a protocol which works for etiolated seedlings, or any suggestions regarding RNA extraction from etiolated material. Thank you in advance, Stefan  |
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| etiolated , extraction , rna , seedlings |
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