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RNA extraction from etiolated seedlings

RNA extraction from etiolated seedlings - Arabidopsis and Plant Biology

RNA extraction from etiolated seedlings - Discuss Arabidopsis and Plant Biology Research.


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Old 07-17-2007, 09:05 AM
Stefan Weinl
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Default RNA extraction from etiolated seedlings



We are in a process to built our own cDNA library and therefore we are
trying to isolate RNA from etiolated seedlings. Up to now we tried
column-based kits (Qiagen and Promega) and used TriFast solution to
isolate RNA but did not receive suitable RNA.
Now we would like to use a classical protocol using phenol and LiCl. We
would be very thankful for a protocol which works for etiolated
seedlings, or any suggestions regarding RNA extraction from etiolated
material.

Thank you in advance,

Stefan


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