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| Hi, everyone, I am studying a T-DNA insertional mutant. It's very interesting that the T-DNA heterozygous lines(Tt) are semi-sterile, but both the T-DNA homozygous lines(TT) and the T-DNA negative lines(tt) are full fertile. the T-DNA is inserted into the 3'UTR of the gene as indicated by KOME full-length cDNA information. However, I found that the gene expression level is not changed in both Tt or TT lines, so it seems that the protein structure may be changed by the insertion and the semi-sterile is the consequence of protein-protein interaction? So anyone can help me to judge the insertion point is really in UTR or coding region? Thanks a lot! Jian |
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| coding , region , utr |
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