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MS Plate Contamination

MS Plate Contamination - Arabidopsis and Plant Biology

MS Plate Contamination - Discuss Arabidopsis and Plant Biology Research.


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Old 04-24-2007, 09:40 PM
Heather Babka
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Default MS Plate Contamination



Hello,

I am trying to select T1 plants on MS plates with 30ug/mL Hygromycin. It
seems that all of this newly collected seed is contaminated with bacteria
(Agro?). Is there anything I can add to the media that would kill the
Agrobacteria, but not harm the plants? Currently I sterilize with 50%
Bleach + 0.02% Triton. I tried adding a 70% Ethanol wash but that did not
help.

Thanks,
Heather

--
Heather L. Babka
Interdepartmental Genetics
Department of Genetics, Development, and Cell Biology
433 Bessey Hall
Iowa State University
Ames, IA 50011
Phone: (515) 294-3509
Fax: (515) 294-1337
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