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#1
04-24-2007, 09:40 PM
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 MS Plate Contamination Hello, I am trying to select T1 plants on MS plates with 30ug/mL Hygromycin. It seems that all of this newly collected seed is contaminated with bacteria (Agro?). Is there anything I can add to the media that would kill the Agrobacteria, but not harm the plants? Currently I sterilize with 50% Bleach + 0.02% Triton. I tried adding a 70% Ethanol wash but that did not help. Thanks, Heather -- Heather L. Babka Interdepartmental Genetics Department of Genetics, Development, and Cell Biology 433 Bessey Hall Iowa State University Ames, IA 50011 Phone: (515) 294-3509 Fax: (515) 294-1337  |
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