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Silwet L-77 addition critical? How important is the detergent Silwet L77 Vac-in-Stuff for Arabidopsis plant transformation? I recently was conducting some plant transformations, but from some miscalculations, I added in too little Silwet and too much water. Will these plants still undergo transformation without Silwet? I made some backup lines just in case, but they are still growing. I'd love to know this answer, as I'm incredibly curious about the use of Silwet. |
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