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Insertion phenotype due to chromosomal translocation??

Insertion phenotype due to chromosomal translocation?? - Arabidopsis and Plant Biology

Insertion phenotype due to chromosomal translocation?? - Discuss Arabidopsis and Plant Biology Research.


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Old 11-17-2006, 08:58 AM
Hongyong Fu
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Default Insertion phenotype due to chromosomal translocation??



Dear all: We recently got a gametophyte defect phenotype (50% abortion)
which is only associated with heterozygous T-DNA insertion. Wow! It looks
like a dominant negative effect associated with insertion! However, before
we are too excited, there are reports indicated that a T-DNA insertion
possibly causes a reciprocal translocation. And, when a homozygous
translocated T-DNA insertion plant crosses to a wild-type plant which will
cause gametophyte abortion in a ratio about 50% (both male and female). Is
there a simple way to rule out our phenotype is not due to reciprocal
translocation of chromosome caused by T-DNA insertion. Thank you for your
advice in advance.



Hongyong Fu

Associate Research Fellow

Institute of Plant and Microbial Biology

Academia Sinica, Taipei, Taiwan

Tel: 886-2-27892392

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