I have been with Arabidopsis protoplast for almost 3 years now, from the
limited experience I have. I think to get good protoplasts and transfection
there are several important points.
1. growth of the plant meterial and the nutrition.
2. Isolation of protoplasts
2. the PEG incubation time and the amount of DNA
but to comment on any of that I need to know what specific things you used
in your experiment.
On 11/13/06, [Only registered users see links. ] < [Only registered users see links. ]> wrote:
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