| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Arabidopsis and Plant Biology Discuss Arabidopsis and Plant Biology Research. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||
| |||
| Hi I have been working with Salk mutant lines obtained from ABRC,and have identified a mutant I'm interested.But PCR analysis using T-DNA border and genomic flanking primers showed insertion site was not as Salk center predicted.So I designed a tail-PCR to recover the flanking sequence myself. I obtained 300bp dna fragment with 200bp LB sequence and 100bp flanking sequence.But when I run blast with the 100bp sequence , result showed this fragment belong to no species,neither arabidopsis nor T-DNA ect..... Could anyone give me some advice about this phenomena?Is this a common problem of failed Tail-PCR? Any advice will be appreciated Yuefeng Guan |
| Tags |
| lines , mutant , problem , salk , tailpcr , unexplanable |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| AtCBF2 salk line mutant | Julio Salinas | Arabidopsis and Plant Biology | 0 | 01-09-2007 04:35 PM |
| AtCBF2 salk line mutant | Dir2005 Yang | Arabidopsis and Plant Biology | 0 | 01-05-2007 10:25 AM |
| AtCBF2 salk line mutant | Luoping Yan | Arabidopsis and Plant Biology | 0 | 01-04-2007 10:46 PM |
| Breast cancer cell lines as models for breast tumors: a review | scimedweb | Cell Biology and Cell Culture | 0 | 02-10-2004 09:21 AM |
| Integration Problem / Finding Contour lines Problem | blah12@mail.com | Physics Forum | 2 | 09-07-2003 11:28 PM |