Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Arabidopsis and Plant Biology
Register Search Today's Posts Mark Forums Read

Arabidopsis and Plant Biology Discuss Arabidopsis and Plant Biology Research.


agar-grown roots. Arab-gen digest, vol 12, issue 2

agar-grown roots. Arab-gen digest, vol 12, issue 2 - Arabidopsis and Plant Biology

agar-grown roots. Arab-gen digest, vol 12, issue 2 - Discuss Arabidopsis and Plant Biology Research.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-07-2006, 10:20 AM
Lorvellec, Maelle
Guest
 
Posts: n/a
Default agar-grown roots. Arab-gen digest, vol 12, issue 2



Message: 1
Date: Fri, 31 Mar 2006 15:42:15 -0500
From: "Redman, Julia C." <[Only registered users see links. ]>
Subject: [Arabidopsis] Agar-grown roots for RNA
To: <[Only registered users see links. ].indiana.edu>
Message-ID:
<7F0AA25C9B1706448E8B73DFDD109B3102453795@EXCHANGE .TIGR.ORG>
Content-Type: text/plain; charset="us-ascii"

Hello everyone,

We're looking to do some expression profiling using root tissue grown in
MS Agar and I was wondering about the logistics of plucking roots from
agar for RNA extraction. What is the best way to go about this without
breaking them?

Any advice will be appreciated!

Julia Redman

The Institute for Genomic Research

Dear Julia,

For isolating RNA, I sow the seeds in 1 ligne on 1 plate of 1/2 MS and I grow them vertically so
the roots grow on top of the agar. Then with a knife I cut all the roots in one time at the
same level and harvest them for RNA.
You can also still grow them horizontaly in 1/2 MS plate and pull them very gently but then they
should not grow for more than a week and you need to cut each root individually.
1/2 MS = 2.2g/L Murashige and Skoog 10 +vitamins, 10 g/L sucrose, pH 5.8 with KOH, 0.8% Diashin agarose.

Hope it helps. If you have more questions about RNA isolation please let me know.

Maƫlle

Laboratory of Molecular Biology.Plant Sciences.Wageningen University.Netherlands.





Reply With Quote
Reply

Tags
agargrown , arabgen , digest , issue , roots , vol


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
[Arabidopsis] brands of agar Forde, Brian Arabidopsis and Plant Biology 1 10-31-2010 08:39 PM
Yeast Digest, Vol 45, Issue 2 Shaoyu Wang Yeast Forum 0 02-05-2009 07:09 PM
[Drosophila] AW: Dros Digest, Vol 46, Issue 2 James Harvey Drosophila Forum 0 02-05-2009 03:04 PM
Zbrafish Digest, Vol 28, Issue 3 raj abi Zebrafish Forum 0 09-07-2007 07:35 PM
Proteins Digest, Vol 22, Issue 15 Dan Guire Protein Forum 0 03-31-2007 10:13 PM


All times are GMT. The time now is 12:55 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11791 seconds with 16 queries