Last year I posted a note, looking for a supplier of TIPS/TNS -
triisopropylnaphthalenesulfonic acid for use in plant RNA preps. This
reagent was especially useful in generating large amounts of
array-quality RNA from etiolated Arabidopsis, among other tissues.
I was deluged with responses stating that this reagent was no longer
available. Some suggested I substitute SDS. This substitution does
not work well.
I was able to locate a supplier of DIPS/DNS, a di-isopropyl
substitute of naphthalenesulfonic acid. Because the downstream
applications are sensitive, time consuming and costly, we extensively
tested DIPS as a substitute for TIPS, even though they are almost
The results of these trials verified that DIPS is a direct
replacement for TIPS in protocols utilizing
I found the reagent at Cactus Biotech in Hayward California,
<http://www.cactusbiotech.com/>[Only registered users see links. ] .