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TNS/TIPS for RNA preparation

TNS/TIPS for RNA preparation - Arabidopsis and Plant Biology

TNS/TIPS for RNA preparation - Discuss Arabidopsis and Plant Biology Research.


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  #1  
Old 04-26-2004, 11:13 PM
Kevin Folta
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Default TNS/TIPS for RNA preparation



Last year I posted a note, looking for a supplier of TIPS/TNS -
triisopropylnaphthalenesulfonic acid for use in plant RNA preps. This
reagent was especially useful in generating large amounts of
array-quality RNA from etiolated Arabidopsis, among other tissues.

I was deluged with responses stating that this reagent was no longer
available. Some suggested I substitute SDS. This substitution does
not work well.

I was able to locate a supplier of DIPS/DNS, a di-isopropyl
substitute of naphthalenesulfonic acid. Because the downstream
applications are sensitive, time consuming and costly, we extensively
tested DIPS as a substitute for TIPS, even though they are almost
identical.

The results of these trials verified that DIPS is a direct
replacement for TIPS in protocols utilizing
alkylnaphthalenesulfonates.

I found the reagent at Cactus Biotech in Hayward California,
<http://www.cactusbiotech.com/>[Only registered users see links. ] .

Thanks for all of your input.

Kevin Folta

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  #2  
Old 04-29-2004, 11:03 PM
Dr. Hiranya S. Roychowdhury
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Default TNS/TIPS for RNA preparation

Thanks for the TIP =

Quoting Kevin Folta <kfolta@mail.ifas.ufl.edu>:



--
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
---
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